PCR vs qPCR: choosing a thermal cycler

Both amplify DNA by thermal cycling, but they answer different questions.

Endpoint PCR

Amplifies a target for downstream use (cloning, sequencing, genotyping). Result is read after cycling, typically on a gel. A standard thermal cycler suffices.

Quantitative PCR (qPCR / real-time)

Measures product accumulation every cycle via fluorescence, enabling absolute or relative quantitation. Requires a real-time instrument with an optical block and dye/probe chemistry (SYBR or TaqMan).

Buying guidance

• Need quantitation, viral load, gene expression → qPCR instrument.
• Only amplification for downstream steps → endpoint cycler (lower cost).
• Check block format (96/384), gradient capability and ramp rate.